HVAC Systems Encyclopedia

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Mold Growth Models

Mold growth prediction models provide quantitative tools for assessing moisture damage risk in building envelopes. These models translate hygrothermal analysis results into biological risk metrics, enabling engineers to evaluate design alternatives and establish performance thresholds for moisture control systems.

VTT Mold Growth Model

The VTT (Technical Research Centre of Finland) model represents the most widely validated empirical approach for mold growth prediction on building materials. Developed by Viitanen and Ojanen, the model calculates a mold index (MI) ranging from 0 to 6 based on time-dependent exposure to temperature and relative humidity conditions.

Model Structure

The VTT model employs differential equations that account for:

Growth Phase:

  • Mold index increases when surface conditions exceed critical thresholds
  • Growth rate depends on material sensitivity class, temperature, and RH
  • Maximum growth rate occurs at optimal conditions (25-30°C, >95% RH)

Decline Phase:

  • Mold index decreases during dry periods
  • Decline rate is slower than growth rate (hysteresis effect)
  • Complete reversal does not occur; residual spores remain viable

Mathematical Formulation:

When conditions favor growth:

dM/dt = (1/7) × (1/t_max) × k₁ × k₂

Where:

  • M = Mold index (0-6 scale)
  • t_max = Time to reach maximum mold index under optimal conditions
  • k₁ = Intensity factor dependent on RH
  • k₂ = Intensity factor dependent on temperature

When conditions inhibit growth:

dM/dt = -0.032 (for M > 1)
dM/dt = 0 (for M ≤ 1)

Mold Index Scale

IndexVisual ObservationPractical Significance
0No growthAcceptable conditions
1Initial spore germination (microscopic)Risk threshold
2Sparse growth (microscopic, <10% coverage)Early detection possible
3Visual growth (<10% coverage)Visible damage begins
4Visual coverage 10-50%Clear deterioration
5Visual coverage 50-100%Extensive damage
6Heavy, profuse growthComplete colonization

Material Sensitivity Classes

The VTT model categorizes materials into four sensitivity classes based on their susceptibility to mold growth:

Very Sensitive Materials (Class 1):

  • Untreated wood (pine sapwood)
  • Porous insulation materials
  • Nutrient-rich surfaces
  • Critical RH threshold: 80%

Sensitive Materials (Class 2):

  • Treated wood products
  • Planed pine surfaces
  • Concrete with biodegradable additives
  • Critical RH threshold: 85%

Medium Resistant Materials (Class 3):

  • Cement-based materials
  • Plaster surfaces
  • Painted wood
  • Critical RH threshold: 90%

Resistant Materials (Class 4):

  • Glass surfaces
  • Glazed ceramic tiles
  • PVC materials
  • Critical RH threshold: 95%

Isopleth Systems

Biohygrothermal isopleths represent graphical models that define boundary conditions for mold germination and growth. These diagrams plot temperature versus relative humidity, with curves indicating germination time and growth rates.

Lowest Isopleth for Mold (LIM)

The LIM curve defines the minimum combined temperature-humidity conditions that permit initial spore germination. Conditions below this threshold prevent mold establishment regardless of exposure duration.

Key LIM Characteristics:

  • Minimum RH decreases as temperature increases (within viable range)
  • At 5°C: approximately 85-90% RH required
  • At 20°C: approximately 80% RH required
  • At 30°C: approximately 78-80% RH required

Isopleth Applications

Isopleth systems serve multiple engineering functions:

  1. Design Verification: Compare hygrothermal simulation results against critical isopleths
  2. Material Selection: Identify materials whose resistance exceeds predicted exposure conditions
  3. Risk Duration Assessment: Estimate time to visible growth under specific conditions
  4. Threshold Definition: Establish control setpoints for active moisture management systems

Time-of-Wetness Models

Time-of-wetness (TOW) approaches simplify mold risk assessment by tracking cumulative duration above critical moisture thresholds. These models assume binary conditions: growth-permitting or growth-inhibiting.

Critical Moisture Thresholds

Material TypeCritical RHCritical MCTemperature Range
Wood products80%20%5-30°C
Gypsum board90%1%10-30°C
Concrete95%5%15-30°C
Insulation (organic)85%15%5-30°C
Painted surfaces90%N/A10-30°C

Accumulation Criteria

TOW models typically employ one of three accumulation methods:

Simple Accumulation:

  • Count hours above critical RH threshold
  • Common criterion: <500 hours/year at surface RH >80%
  • Does not account for temperature variation

Degree-Hour Method:

  • Weight hours by RH excess above threshold
  • Formula: Σ(RH - RH_critical) × time
  • Provides severity weighting

Temperature-Adjusted TOW:

  • Apply multipliers based on temperature favorability
  • Growth factor at 25°C = 1.0
  • Growth factor at 10°C ≈ 0.3
  • Growth factor at 5°C ≈ 0.1

ASHRAE Standard 160

ASHRAE 160 (Criteria for Moisture-Control Design Analysis in Buildings) establishes quantitative performance criteria for preventing mold growth in building assemblies.

30-Day Running Average Method

ASHRAE 160 employs a 30-day moving average of surface RH and temperature to assess mold growth risk. This approach filters short-term fluctuations while capturing sustained moisture exposure.

Acceptance Criteria:

Surface TypeMaximum 30-Day Avg RHTemperature Condition
Sensitive materials80%All temperatures
Intermediate materials85%T > 5°C
Resistant materials90%T > 10°C

Implementation Requirements

Simulation Parameters:

  • Minimum 3-year hourly simulation required
  • Use typical meteorological year (TMY) climate data
  • Include solar radiation and wind-driven rain
  • Model actual construction sequence and drying periods

Evaluation Locations:

  • All material interfaces subject to condensation risk
  • Exterior sheathing surfaces
  • Interior vapor retarder surfaces
  • Roof deck assemblies
  • Foundation walls and slabs

Pass/Fail Criteria:

  • No 30-day period shall exceed material-specific RH thresholds
  • Initial construction moisture must dry below criteria within first year
  • Analysis must demonstrate compliance for worst-case orientation

ESP-r Mold Prediction Module

The ESP-r building simulation platform incorporates a deterministic mold growth model based on substrate-specific germination and growth rate functions.

Model Features:

  • Hourly calculation of germination potential
  • Species-specific parameters (Aspergillus, Penicillium, Stachybotrys)
  • Integration with hygrothermal solver
  • Surface and interstitial prediction capability

Critical Parameters:

  • Minimum germination time: 24-72 hours at optimal conditions
  • Decay rate during dry periods: 50% reduction per 30 days
  • Temperature optimum: 25-28°C for most species
  • Substrate adjustment factors for nutrient availability

Material-Specific Growth Characteristics

Different building materials exhibit distinct mold growth behaviors due to variations in nutrient content, pH, surface texture, and moisture retention.

Wood and Wood-Based Products

Growth Factors:

  • Fiber saturation point: ~28% moisture content
  • Surface mold initiation: 20-22% MC
  • Deep colonization: >25% MC sustained
  • Species variation: sapwood > heartwood susceptibility

Critical Conditions:

  • Germination threshold: 80% RH at 20°C for 5-7 days
  • Visible growth: 85% RH at 20°C for 30 days
  • Temperature acceleration: growth rate doubles per 10°C rise (5-30°C range)

Gypsum-Based Materials

Growth Factors:

  • Paper facing provides primary nutrient source
  • Core remains relatively resistant
  • Capillary moisture transport from adjacent materials
  • Alkalinity inhibits growth initially

Critical Conditions:

  • Surface RH threshold: 90% at 25°C
  • Paper backing saturation: critical risk point
  • Repeated wetting cycles: cumulative damage
  • Contact with wet materials: direct moisture transfer

Concrete and Masonry

Growth Factors:

  • High initial pH (12-13) inhibits growth
  • Carbonation reduces alkalinity over time
  • Surface dust and efflorescence provide nutrients
  • Porous structure retains moisture

Critical Conditions:

  • New concrete: minimal risk for 6-12 months
  • Aged concrete: 95% RH threshold at 20°C
  • Surface condensation: primary initiation mechanism
  • Thermal bridging locations: elevated risk zones

Model Validation and Uncertainty

Mold growth models carry inherent uncertainties due to biological variability and environmental complexity.

Validation Studies

Laboratory Validation:

  • Controlled exposure chambers with inoculated specimens
  • Measured agreement: ±0.5 mold index units
  • Temperature range: 5-30°C
  • RH range: 75-100%

Field Validation:

  • Limited full-scale validation data available
  • Correlation with observed damage: moderate to good
  • Under-prediction common in optimal growth conditions
  • Over-prediction in borderline conditions

Uncertainty Sources

  1. Material Variability: Substrate composition, surface condition, contamination
  2. Biological Variation: Species diversity, spore availability, adaptation
  3. Microclimate Effects: Air film resistance, local convection, radiation
  4. Model Simplifications: Nutrient depletion, competition, succession ignored

Safety Factors

Conservative design practice applies safety margins:

  • Reduce critical RH thresholds by 5% for design purposes
  • Apply 1.5× factor to predicted TOW accumulation
  • Consider worst-case material sensitivity classification
  • Evaluate multiple climate years for inter-annual variability

Integration with Hygrothermal Analysis

Mold growth models require accurate hygrothermal boundary conditions from coupled heat and moisture transport simulations.

Required Inputs

Environmental Conditions:

  • Hourly outdoor temperature and RH
  • Solar radiation intensity and angle
  • Wind speed and direction
  • Precipitation (wind-driven rain)

Material Properties:

  • Moisture storage functions
  • Liquid and vapor transport coefficients
  • Thermal conductivity (moisture-dependent)
  • Initial moisture content

Boundary Conditions:

  • Interior temperature and RH schedules
  • Surface heat transfer coefficients
  • Air leakage rates through assembly
  • Solar absorptance and emittance

Simulation Best Practices

  1. Mesh Refinement: Fine spatial discretization at material interfaces (≤5 mm)
  2. Time Step: Hourly maximum for mold prediction (1-15 min for hygrothermal core)
  3. Convergence: Verify moisture balance closure (<1% error)
  4. Duration: Minimum 3 years to capture inter-annual variability
  5. Post-Processing: Apply moving averages to filter numerical noise

Accurate mold growth prediction enables evidence-based envelope design decisions, quantifies moisture safety margins, and establishes performance acceptance criteria for moisture control strategies. Engineers must select models appropriate to material systems, validate predictions against acceptance criteria, and apply conservative thresholds to account for model uncertainties and field variability.

Sections

VTT Model

Finnish VTT mold growth model for predicting mold risk in building assemblies based on time-of-wetness method, mold index calculations, sensitivity classes, and critical thresholds per ASHRAE 160 implementation.

Isopleth Systems

Isopleth systems provide graphical representation of mold growth boundaries as functions of temperature, relative humidity, and time. These systems originated from mycological research by Ayerst (1969) and have been expanded by researchers including Sedlbauer (2001) to create comprehensive mold prediction frameworks.

Fundamental Principles

An isopleth represents a line on a temperature-humidity diagram connecting points of equal biological activity. In mold growth prediction, isopleths delineate boundaries between conditions that support or inhibit fungal development.

Biohygrothermal Models

Biohygrothermal models couple mold growth predictions with dynamic heat and moisture transport simulations in building assemblies. These models predict mold development under transient boundary conditions by solving simultaneous differential equations for temperature, moisture, and biological activity.

VTT Mold Growth Model

Model Structure

The VTT model (Technical Research Centre of Finland) integrates biological growth kinetics with material hygrothermal properties.

Mold Index Evolution:

dM/dt = (1/7) × k₁ × k₂ × exp(-0.68 ln(RHcrit) - 13.9 ln(RHcrit)/T + 0.14 W - 0.33 ln(t₁))

Where:

ASHRAE 160 Standard

ASHRAE Standard 160 provides design criteria for moisture control in buildings to prevent mold growth and material deterioration. The standard establishes surface relative humidity limits and temperature-RH combinations that predict conditions favorable for mold development.

Standard Scope and Application

ASHRAE 160 applies to:

  • Residential buildings
  • Commercial buildings
  • Institutional facilities
  • New construction and major renovations
  • All climate zones

The standard addresses moisture control during both design and construction phases.

Design Criteria Philosophy

ASHRAE 160 establishes performance-based criteria: